Velocity differences between L- and P-selectin-dependent neutrophil rolling in venules of mouse cremaster muscle in vivo

被引:80
作者
Jung, U
Bullard, DC
Tedder, TF
Ley, K
机构
[1] UNIV VIRGINIA, HLTH SCI CTR, SCH MED, DEPT BIOMED ENGN, CHARLOTTESVILLE, VA 22908 USA
[2] BAYLOR COLL MED, DEPT MOL & HUMAN GENET, HOUSTON, TX 77030 USA
[3] DUKE UNIV, MED CTR, DEPT IMMUNOL, DURHAM, NC 27710 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1996年 / 271卷 / 06期
关键词
leukocyte rolling; granulocytes; surgical trauma;
D O I
10.1152/ajpheart.1996.271.6.H2740
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
After surgical trauma, leukocyte rolling is initially normal in L-selectin-deficient mice and reduced at later times, whereas leukocyte rolling is initially absent in P-selectin-deficient mice but induced later. Here, we examined the possibility that P- and L-selectin support foiling at different characteristic velocities using intravital microscopy of venules of the exteriorized cremaster muscle venules of wild type (WT) and P- and L-selectin-deficient mice. At >50 min after exteriorization, rolling in P-selectin-deficient mice occurred at significantly higher velocities (129 +/- 89 mu m/s) than in WT mice (49 +/- 23 mu m/s). Rolling velocity distribution in L-selectin-deficient mice was similar to WT mice immediately after exteriorization. Histological examination of Giemsa-stained whole-mount preparations in cremaster muscle venules revealed that the majority of rolling cells (similar to 90% in all genotypes) were granulocytes. We conclude that P-selectin mediates leukocyte rolling at velocities <50 mu m/s, whereas L-selectin sustains more rapid rolling. Under physiological conditions, P- and L-selectin synergize to support rolling at velocities between 20 and 70 mu m/s as seen in WT mice.
引用
收藏
页码:H2740 / H2747
页数:8
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