Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system

被引：64

作者：

Ajayi, T

Allmond, LR

Sawa, T

Wiener-Kronish, JP

机构：

[1] Univ Calif San Francisco, Dept Anesthesia, Div Pulm & Crit Care Med, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Dept Anesthesia & Perioperat Care, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94143 USA

[4] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 08期

关键词：

D O I：

10.1128/JCM.41.8.3526-3531.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and specificity in this small sample size. This assay appears to be promising for the rapid and accurate genotyping of the presence of these genes in clinical strains of P. aeruginosa.

引用

页码：3526 / 3531

页数：6

共 41 条

[1] Acquisition of expression of the Pseudomonas aeruginosa ExoU cytotoxin leads to increased bacterial virulence in a murine model of acute pneumonia and systemic spread [J].