Brassinosteroid levels increase drastically prior to morphogenesis of tracheary elements

被引:116
作者
Yamamoto, R [1 ]
Fujioka, S
Demura, T
Takatsuto, S
Yoshida, S
Fukuda, H
机构
[1] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo, Japan
[2] RIKEN, Inst Phys & Chem Res, Wako, Saitama 3510198, Japan
[3] Joetsu Univ Educ, Dept Chem, Niigata 9438512, Japan
关键词
D O I
10.1104/pp.125.2.556
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary Bird) cells differentiating into tracheary elements. The results of feeding experiments suggested that both the early and late C6-oxidation pathways occur during tracheary element differentiation. Gas chromatography-mass spectrometry analysis revealed that five BRs, castasterone, typhasterol, 6-deoxocastasterone, 6-deoxotyphasterol, and 6-deoxoteasterone, actually existed in cultured zinnia cells and culture medium. Quantification of endogenous BRs in each stage of tracheary element differentiation by gas chromatography-mass spectrometry exhibited that they increased dramatically prior to the morphogenesis, which was consistent with the idea that BRs are necessary for the initiation of the final stage of tracheary element differentiation. Moreover, the proportion of each BR in culture medium was quite different from that in cells, suggesting that specific BRs are selectively secreted into medium and may function outside the cells.
引用
收藏
页码:556 / 563
页数:8
相关论文
共 39 条
[1]   Molecular physiology of brassinosteroids revealed by the analysis of mutants [J].
Altmann, T .
PLANTA, 1999, 208 (01) :1-11
[2]   Brassinosteroid biosynthesis inhibitors [J].
Asami, T ;
Yoshida, S .
TRENDS IN PLANT SCIENCE, 1999, 4 (09) :348-353
[3]   STEROL BIOSYNTHESIS [J].
BENVENISTE, P .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1986, 37 :275-308
[4]   The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis [J].
Bishop, GJ ;
Nomura, T ;
Yokota, T ;
Harrison, K ;
Noguchi, T ;
Fujioka, S ;
Takatsuto, S ;
Jones, JDG ;
Kamiya, Y .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (04) :1761-1766
[5]   PLANT CYTOCHROME-P450 [J].
BOLWELL, GP ;
BOZAK, K ;
ZIMMERLIN, A .
PHYTOCHEMISTRY, 1994, 37 (06) :1491-1506
[6]   Molecular-genetic analysis of plant cytochrome P450-dependent monooxygenases [J].
Chapple, C .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1998, 49 :311-343
[7]   The Arabidopsis dwf7/ste1 mutant is defective in the Δ7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis [J].
Choe, SW ;
Noguchi, T ;
Fujioka, S ;
Takatsuto, S ;
Tissier, CP ;
Gregory, BD ;
Ross, AS ;
Tanaka, A ;
Yoshida, S ;
Tax, FE ;
Feldmann, KA .
PLANT CELL, 1999, 11 (02) :207-221
[8]   The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22α-hydroxylation steps in brassinosteroid biosynthesis [J].
Choe, SW ;
Dilkes, BP ;
Fujioka, S ;
Takatsuto, S ;
Sakurai, A ;
Feldmann, KA .
PLANT CELL, 1998, 10 (02) :231-243
[9]  
Choi YH, 1997, PHYTOCHEMISTRY, V44, P609, DOI 10.1016/S0031-9422(96)00572-9
[10]  
Clouse S. D., 1991, Brassinosteroids. Chemistry, bioactivity and applications., P122