DNA methylation profiling using the methylated-CpG island recovery assay (MIRA)

被引：42

作者：

Rauch, Tibor A. ^{[2
]}

Pfeifer, Gerd P. ^{[1
]}

机构：

[1] City Hope Natl Med Ctr, Div Biol, Beckman Res Inst, Duarte, CA 91010 USA

[2] Rush Univ, Sect Mol Med, Dept Orthoped Surg, Med Ctr, Chicago, IL 60612 USA

来源：

METHODS | 2010年 / 52卷 / 03期

关键词：

DNA methylation; CpG islands; Epigenome; Microarrays; High-throughput sequencing; LUNG-CANCER; RESOLUTION; PROTEINS; CELLS; WIDE;

D O I：

10.1016/j.ymeth.2010.03.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The methylated-CpG island recovery assay (MIRA) exploits the intrinsic specificity and the high affinity of a methylated-CpG-binding protein complex (MBD2B and MBD3L1) to methylated CpG dinucleotides in genomic DNA. The MIRA approach works on double-stranded DNA and does not depend on the application of methylation-sensitive restriction enzymes. It can be performed on a few hundred nanograms of genomic DNA. Recently, the MIRA technique has been used to profile DNA methylation patterns at a resolution of 100 base pairs along the entire genome of normal human B-lymphocytes. The MIRA method is compatible with microarray and next generation DNA sequencing approaches. We describe the principles and details of this method applied for methylation profiling of genomes containing methylated CpG sequences. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：213 / 217

页数：5

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