Preliminary x-ray crystallographic analysis of centrin from ciliate Euplotes octocarinatus

被引：1

作者：

He, XJ

Li, L

Hou, H

Zhao, YQ

Liang, AH

Rao, ZH ^{[1
]}

机构：

[1] Tsinghua Univ, Struct Biol Lab, Beijing 100084, Peoples R China

[2] Chinese Acad Sci, China & Natl Lab Macromol, Inst Biophys, Beijing 100101, Peoples R China

[3] Shanxi Univ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Minist Educ, Yaiyuan 030006, Peoples R China

来源：

PROTEIN AND PEPTIDE LETTERS | 2005年 / 12卷 / 06期

关键词：

centrin; crystallization; preliminary X-ray studies;

D O I：

10.2174/0929866054395842

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 angstrom. The crystals belong to space group P2(1)2(1)2(1) and the unit-cell parameters are a=34.442 angstrom, b=48.954 angstrom, c=72.583 angstrom.

引用

页码：609 / 611

页数：3

共 18 条

[1]

Andersen SSL, 1999, INT REV CYTOL, V187, P51

[2] YEAST GENE REQUIRED FOR SPINDLE POLE BODY DUPLICATION - HOMOLOGY OF ITS PRODUCT WITH CA-2+-BINDING PROTEINS [J].