Temporal and spatial expression of matrix metalloproteinases during wound healing of human corneal tissue

被引:71
作者
Daniels, JT
Geerling, G
Alexander, RA
Murphy, G
Khaw, PT
Saarialho-Kere, U
机构
[1] Inst Ophthalmol, Div Pathol, Wound Healing Res Unit, Epithelial Repair & Regenerat Grp, London EC1V 9EL, England
[2] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England
[3] Univ Helsinki, Dept Dermatol & Venerol, FIN-00014 Helsinki, Finland
基金
芬兰科学院; 英国惠康基金;
关键词
corneal wound healing; metalloproteinase; temporal modulation; laminin-5; TIMP-1; gelatinase;
D O I
10.1016/j.exer.2003.08.010
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Our understanding of MMP expression during corneal repair has previously relied upon animal models, isolated human biopsy specimens and cell culture studies. The aim of this study was to determine the temporal and spatial expression of matrix metalloproteinases following wounding of cultured human corneal tissue. Human corneas were cultured and cut into six pieces. The epithelium was removed with a corneal brush. The tissue was then re-cultured and tissue pieces were fixed up to 7 days post-wounding. Matrix metalloproteinases were detected by in situ hybridisation and immunohistochemistry. Intracellular laminin-5, a marker of migratory epithelial cells, was located immunohistologically. In the time scale studied tissue series from nine corneas achieved coverage of the stroma with epithelial cells and partial repair of damaged basement membrane, demonstrated by the Periodic acid-Schiff reaction and haematoxylin and eosin counter-staining. By day 3, migrating epithelial cells and stromal cells beneath the wounded area expressed collagenase-1 (MMP-1). Stromelysin-1 (MMP-3) was expressed only by fibroblast-like stromal cells. Stromelysin-2 (MMP-10) was detected in migrating epithelial cells and remained when the stroma was surrounded by a monolayer of epithelial cells. By day 7, development of multi-layered epithelium around the tissue coincided with cessation of MMP expression in both epithelial and stromal cells, except for MMP-9, which remained in epithelial basal cells. Tissue inhibitor of matrix metalloproteinase-1 was mainly associated with stromal cells and was reduced upon formation of a multilayered epithelium. This study demonstrates matrix metalloproteinase expression in epithelial and fibroblast-like cells following wounding of human corneal tissue in culture where the cells remain in contact with their natural matrices. (C) 2003 Elsevier Ltd. All fights reserved.
引用
收藏
页码:653 / 664
页数:12
相关论文
共 68 条
[1]   Urokinase plasminogen activator is expressed by basal keratinocytes before interstitial collagenase, stromelysin-1, and laminin-5 in experimentally induced dermatitis herpetiformis lesions [J].
Airola, K ;
Reunala, T ;
Salo, S ;
SaarialhoKere, UK .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1997, 108 (01) :7-11
[2]  
Akpek EK, 1997, OPHTHALMOLOGY, V104, P1863, DOI 10.1016/S0161-6420(97)30015-3
[3]  
ALFONSO AA, 1999, INVEST OPHTH VIS SCI, V40, P2506
[4]  
APPLE DJ, 1985, OCULAR PATHOLOGY CLI, P63
[5]  
Azar DT, 1996, CORNEA, V15, P18
[6]   SMOOTH-MUSCLE CELL-MIGRATION AND MATRIX METALLOPROTEINASE EXPRESSION AFTER ARTERIAL INJURY IN THE RAT [J].
BENDECK, MP ;
ZEMPO, N ;
CLOWES, AW ;
GALARDY, RE ;
REIDY, MA .
CIRCULATION RESEARCH, 1994, 75 (03) :539-545
[7]   MATRIX METALLOPROTEINASES - A REVIEW [J].
BIRKEDALHANSEN, H ;
MOORE, WGI ;
BODDEN, MK ;
WINDSOR, LJ ;
BIRKEDALHANSEN, B ;
DECARLO, A ;
ENGLER, JA .
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 1993, 4 (02) :197-250
[8]   PROTEOLYTIC REMODELING OF EXTRACELLULAR-MATRIX [J].
BIRKEDALHANSEN, H .
CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (05) :728-735
[9]  
BREWITT H, 1979, ACTA OPHTHALMOL, V57, P945
[10]  
Crewe JM, 2001, INVEST OPHTH VIS SCI, V42, P1757