Oxidation and generation of hydrogen peroxide by thiol compounds in commonly used cell culture media

被引:73
作者
Long, LH [1 ]
Halliwell, B [1 ]
机构
[1] Natl Univ Singapore, Fac Med, Dept Biochem, Singapore 119260, Singapore
关键词
hydrogen peroxide; GSH; thiol; cysteine; cysteamine; cell culture; homocysteine; autoxidation;
D O I
10.1006/bbrc.2001.5514
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many studies have examined the effects of thiol compounds upon cells in culture (e.g., upon signal transduction and regulation of gene expression), but few have considered how thiols can interact with cell culture media. A wide range of thiols (cysteine, GSH, N-acetylcysteine, gamma -glutamylcysteine, cysteinylglycine, cysteamine, homocysteine) were found to interact with three commonly used cell culture media (RPMI, MEM, DMEM) to generate hydrogen peroxide with complex concentration-dependencies. Thiols added to these media rapidly disappeared, although less H2O2 was generated on a molar basis than the amount of thiol lost. Studies on cellular effects of thiols, especially those on redox regulation of gene expression or protein function, need to take into account that thiols are rapidly lost, and that their oxidation generates H2O2, which can have multiple concentration-dependent effects on cell metabolism. (C) 2001 Academic Press.
引用
收藏
页码:991 / 994
页数:4
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