Time to detection of Mycobacterium tuberculosis as an alternative to quantitative cultures

被引：36

作者：

Bark, C. M. ^{[1
,2
]}

Okwera, A. ^{[3
,4
]}

Joloba, M. L. ^{[4
]}

Thiel, B. A. ^{[2
]}

Nakibali, J. G. ^{[3
,4
]}

Debanne, S. M. ^{[5
]}

Boom, W. H. ^{[2
,4
]}

Eisenach, K. D. ^{[6
,7
]}

Johnson, J. L. ^{[2
,4
]}

机构：

[1] Univ Hosp Case Med Ctr, Div Infect Dis & HIV Med, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Dept Med, TB Res Inst, Cleveland, OH 44106 USA

[3] Ugandan Natl TB & Leprosy Programme, Kampala, Uganda

[4] Uganda Case Western Reserve Univ Res Collaborat, Kampala, Uganda

[5] Case Western Reserve Univ, Sch Med, Dept Epidemiol & Biostat, Cleveland, OH USA

[6] Univ Arkansas Med Sci, Dept Pathol, Little Rock, AR 72205 USA

[7] Univ Arkansas Med Sci, Dept Microbiol, Little Rock, AR 72205 USA

来源：

TUBERCULOSIS | 2011年 / 91卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Tuberculosis; Time to detection; Colony forming units; BACTEC; EARLY BACTERICIDAL ACTIVITY; PULMONARY TUBERCULOSIS; ADULTS;

D O I：

10.1016/j.tube.2011.01.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Testing new drugs is critical to improving the treatment of tuberculosis. Quantitative cultures of Mycobacterium tuberculosis on solid media have been used in Phase 1 and 2 trials, but are time and resource intensive. Time to detection (TTD) of growth of M. tuberculosis in automated liquid culture systems is an alternative. TTD has been shown to correlate with CFU in quantitative cultures, and is faster and simpler to perform. We compared TTD in the BACTEC 460 liquid culture system with CFU in a clinical trial that included 110 subjects. Comparing all sputum cultures collected between baseline and 2 months we found a strong negative correlation between log(10) CFU and TTD (rho = -0.91). In addition, when TTD at baseline was compared with 1 and 2 month sputum culture positivity, subjects whose cultures were negative after 1 and 2 months had a significantly longer median baseline TTD compared with subjects whose cultures were positive at 1 and 2 months (5 vs. 3 days and 3 vs. 2 days, respectively). TTD compares closely with CFU and represents a faster, simpler alternative to quantitative cultures. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：257 / 259

页数：3

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