Multiphoton excited fluorescence spectroscopy of biomolecular systems

被引:50
作者
Birch, DJS [1 ]
机构
[1] Univ Strathclyde, Dept Phys & Appl Phys, Photophys Res Grp, Glasgow G4 0NG, Lanark, Scotland
基金
英国工程与自然科学研究理事会; 英国惠康基金;
关键词
multiphoton excitation; fluorescence spectroscopy; fluorescence lifetimes; fluorescence anisotropy; biomolecules; membranes; alkanes; proteins; tryptophan; green fluorescent protein; melanin;
D O I
10.1016/S1386-1425(01)00487-5
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Recent work on the emerging application of multiphoton excitation to fluorescence studies of biomolecular dynamics. and structure is reviewed, The fundamental principles and experimental techniques of multiphoton excitation are outlined, fluorescence lifetimes, anisotropy and spectra in membranes, proteins, hydrocarbons, skin, tissue and metabolites are featured, and future opportunities are highlighted. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:2313 / 2336
页数:24
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