Production of conditionally immortalised cell lines from a transgenic mouse

被引：34

作者：

Holley, MC

Lawlor, PW

机构：

[1] Department of Physiology, School of Medical Sciences, University of Bristol

来源：

AUDIOLOGY AND NEURO-OTOLOGY | 1997年 / 2卷 / 1-2期

关键词：

cochlear; vestibular; hair cells; transgenic; Immortomouse; conditional immortalisation;

D O I：

10.1159/000259227

中图分类号：

R36 [病理学]; R76 [耳鼻咽喉科学];

学科分类号：

100104 ; 100213 ;

摘要：

This review describes the H2k(b)tsA58 transgenic mouse (Immortomouse) and its application to the production of conditionally immortalised cell lines from sensory epithelia within the mammalian inner ear. Established cell lines should overcome many of the technical difficulties associated with experimental procedures in auditory and vestibular research. These include the limited amount of tissue available and the relatively complex and laborious dissection. Conditional immortalisation should also allow essential studies on the molecular and cellular mechanisms that govern both the differentiation of sensory cells and the development of sensory epithelia.

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页码：25 / 35

页数：11

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