Expression, mapping, and genetic variability of Brassica napus disease resistance gene analogues

被引：40

作者：

Fourmann, M

Charlot, F

Froger, N

Delourme, R

Brunel, D

机构：

[1] INRA, Genet & Ameliorat Plantes Stn, F-78026 Versailles, France

[2] INRA, Stn Ameliorat Plantes, F-35653 Le Rheu, France

来源：

GENOME | 2001年 / 44卷 / 06期

关键词：

RGAs; Brassica; NBS-LRR; resistance; molecular markers;

D O I：

10.1139/gen-44-6-1083

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Numerous sequences analogous to resistance (R) genes exist in plant genomes and could be involved in resistance traits. The aim of this study was to identify a large number of Brassica napus sequences related to R genes and also to test the adequacy of specific PCR-based tools for studying them. Different consensus primers were compared for their efficiency in amplifying resistance-gene analogues (RGAs) related to the nucleotide-binding-site subgroup of R genes. Specific primers were subsequently designed to fine-study the different RGAs and we tested their efficiency in three species related to B. napus: Brassica oleracea, Brassica rapa, and Arabidopsis thaliana. Forty-four B. napus RGAs were identified. Among 29 examined, at least one-third were expressed. Eighteen RGAs were mapped on 10 of the 19 B. napus linkage groups. The high variability within these sequences permitted discrimination of each genotype within a B. napus collection. The RGA-specific primers amplified RGAs in the B. oleracea and B. rapa genomes, but the sequences appear to be poorly conserved in A. thaliana. Specific RGA primers are a precise tool for studying known-sequence RGAs. These sequences represent interesting markers that could be correlated with resistance traits in B. napus or related Brassica genomes.

引用

页码：1083 / 1099

页数：17

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