Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter haemolyticus, strain ATCC 17906

被引：29

作者：

Haseley, SR

Holst, O

Brade, H

机构：

[1] Division of Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Borstel

[2] Research Centre Borstel, D-23845 Borstel

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 244卷 / 03期

关键词：

Acinetobacter; lipopolysaccharide; O-antigen; alanine;

D O I：

10.1111/j.1432-1033.1997.00761.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polysaccharide containing 2-acetamido-2-deoxy-D-galacturonic acid (GalNAcA), 2,4-diacetamido-2,4,6-trideoxy-D-glucose (QuiNAc4NAc), and D-alanine (Ala) was isolated from the water-soluble lipopolysaccharide (LPS) originating from the reference strain for Acinetobacter haemolyticus (DNA group 4) strain ATCC 17906. The polysaccharide, characterised by means of monosaccharide analyses and NMR studies, was shown to be based on a linear trisaccharide repeating unit, as shown below, with the alanine group amide-bound to position 6 of one GalNAcA residue. It was specifically recognised in western blots by polyclonal rabbit antisera. [GRAPHICS]

引用

页码：761 / 766

页数：6

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