Characterization of exopolysaccharides produced by 19 halophilic strains of the species Halomonas eurihalina

被引:86
作者
Béjar, V [1 ]
Llamas, I
Calvo, C
Quesada, E
机构
[1] Univ Granada, Fac Pharm, Dept Microbiol, Exopolysaccharide Res Grp, E-18071 Granada, Spain
[2] Univ Granada, Water Inst, E-18071 Granada, Spain
关键词
Halomonas eurihalina; exopolysaccharides; halophilic bacteria;
D O I
10.1016/S0168-1656(98)00024-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The formation, chemical composition and theological properties of the exopolysaccharides (EPS) produced by 19 strains belonging to Halomonas eurihalina have been compared in two different culture media. Our aim was to screen several strains isolated from saline soils to select those producing maximum EPS yield and good theological properties. We found that MY medium was best for the production of EPS in all the strains studied. Maximum EPS production was 1.6 g l(-1) with strain H212 grown in this medium. The pattern of the chemical composition of the polysaccharides was affected by the strain in question and by the culture medium. All EPS studied had an unusually high sulphate content. Furthermore, the exopolymer from strain H96 contained significant amounts of uronic acid. EPS from strain H96, cultivated in defined NH medium, behaved in an interesting way theologically; when the pH of the polymer solution was decreased to 3.0 a gel with a viscosity of 30 000 cP formed. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:135 / 141
页数:7
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