Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma

被引：53

作者：

Scintu, Marina

Vitale, Rita

Prencipe, Maria

Gallo, Antometta Pia

Bonghi, Loriana

Valori, Vanna Maria

Maiello, Evaristo

Rinaldi, Monica

Signori, Emanuela

Rabitti, Carla

Carella, Massimo

Dallapiccola, Bruno

Altomare, Vittorio

Fazio, Vito M.

Parrella, Paola

机构：

[1] IRCCS Casa Sollievo Sofferenza, Res Dept, Lab Oncol, I-71013 San Giovanni Rotondo, Italy

[2] Univ Bari, Dept Med Biochem Biol & Phys, I-70124 Bari, Italy

[3] IRCCS Casa Sollievo Sofferenza, Dept Oncol, I-71013 San Giovanni Rotondo, Italy

[4] CNR, Inst Neurobiol & Mol Med, Rome, Italy

[5] Mol Med & Biotechnol Lab, I-00155 Rome, Italy

[6] Dept HystoPathol, Rome, Italy

[7] IRCCS Casa Sollievo Sofferenza, Med Genet Lab, I-71013 San Giovanni Rotondo, Italy

[8] Dept Surg, Breast Unit, I-00168 Rome, Italy

来源：

CANCER LETTERS | 2007年 / 254卷 / 02期

关键词：

breast cancer; genomic instability; MAD2L1; BUB1B; gene expression;

D O I：

10.1016/j.canlet.2007.03.021

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUBIB and MAD2L1. All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24-76.78%) than in controls (average: 11.54%, range: 9.91-14.84%) (P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34-35.8%) as compared with controls (average: 4.18% range: 3.47-4.81%) (P < 0.0001). An increase in BUBIB and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUBIB mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability (r = 0.722, P = 0.018). (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：298 / 307

页数：10

共 44 条

[1] Mechanisms of aneuploidy in thyroid cancer cell lines and tissues:: evidence for mitotic checkpoint dysfunction without mutations in BUB1 and BUBR1 [J].