Abdominal wall repair using a biodegradable scaffold seeded with cells

被引:42
作者
Drewa, T [1 ]
Galazka, P
Prokurat, A
Wolski, Z
Sir, J
Wysocka, K
Czajkowski, R
机构
[1] L Rydygier Med Univ, Dept Med Biol, PL-85094 Bydgoszcz, Poland
[2] L Rydygier Med Univ, Dept Urol, PL-85094 Bydgoszcz, Poland
[3] L Rydygier Med Univ, Dept Pediat Surg, PL-85094 Bydgoszcz, Poland
[4] F Lukaszczyk Oncol Ctr, Dept Pathol, PL-85094 Bydgoszcz, Poland
关键词
tissue engineering; abdominal wall defect; reconstruction; animal model;
D O I
10.1016/j.jpedsurg.2004.10.019
中图分类号
R72 [儿科学];
学科分类号
100202 [儿科学];
摘要
Background/Purpose: The repair of large abdominal wall defects is still a challenge for pediatric surgeons. Synthetic materials, however, may lead to high complication rates. This study was aimed at applying tissue-engineering methods to abdominal wall repair. Methods: 3T3 mouse fibroblasts were expanded in vitro. In the next step, a biodegradable material polyglycolic acid (PGA)-was actively seeded with 10(7) cells/sem of PGA scaffold. Culture medium (Dulbecco's Modified Eagle's Medium with 10% fetal bovine serum) was changed every 6 hours after seeding cells on PGA fibers. Under general anaesthesia, C57BL/6J black mice underwent creation of a 2 x 3-cm abdominal wall defect (60%-70% of abdominal surface). The defect was repaired in the experimental group with the fibroblast-seeded PGA scaffold. In the first control group, the defect was covered with acellular PGA, and in the second control group, by skin closure. Animals were killed after 30 days to assess the histologic and gross findings. Results: No abdominal hernia was found in animals repaired with cell-seeded and acellular scaffolds. All animals with skin closure died within 7 days. In every case, tissue-engineered construct was thicker then in controls. Histologic and gross examination revealed a good neovascularisation in tissue engineered abdominal walls comparing to the acellular matrix. There was no intensive scar formation between abdominal wall and skin. Conclusions: Engineered soft tissue constructs can provide structural replacement of severe and large abdominal wall defects. Tissue engineering in the near future will possibly enter clinical practice. (C) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:317 / 321
页数:5
相关论文
共 17 条
[1]
Evaluation of small intestine submucosa and acellular dermis as diaphragmatic prostheses [J].
Dalla Vecchia, LD ;
Engum, S ;
Kogon, B ;
Jensen, E ;
Davis, M ;
Grosfeld, J .
JOURNAL OF PEDIATRIC SURGERY, 1999, 34 (01) :167-171
[2]
Unsatisfactory experience with 'minimal intervention management' for gastroschisis [J].
Dolgin, SE ;
Midulla, P ;
Shlasko, E .
JOURNAL OF PEDIATRIC SURGERY, 2000, 35 (10) :1437-1439
[3]
Fetal tissue engineering: Diaphragmatic replacement [J].
Fauza, DO ;
Marler, JJ ;
Koka, R ;
Forse, RA ;
Mayer, JE ;
Vacanti, JP .
JOURNAL OF PEDIATRIC SURGERY, 2001, 36 (01) :146-151
[4]
GASTROSCHISIS - A SIMPLE TECHNIQUE FOR STAGED SILO CLOSURE [J].
FISCHER, JD ;
CHUN, K ;
MOORES, DC ;
ANDREWS, HG .
JOURNAL OF PEDIATRIC SURGERY, 1995, 30 (08) :1169-1171
[5]
Fetal tissue engineering: In vitro analysis of muscle constructs [J].
Fuchs, JR ;
Pomerantseva, I ;
Ochoa, ER ;
Vacanti, JP ;
Fauza, DO .
JOURNAL OF PEDIATRIC SURGERY, 2003, 38 (09) :1348-1353
[6]
Fetal tissue engineering: Chest wall reconstruction [J].
Fuchs, JR ;
Terada, S ;
Hannouche, D ;
Ochoa, ER ;
Vacanti, JP ;
Fauza, DO .
JOURNAL OF PEDIATRIC SURGERY, 2003, 38 (08) :1188-1193
[7]
Engineered fetal cartilage: Structural and functional analysis in vitro [J].
Fuchs, JR ;
Terada, S ;
Hannouche, D ;
Ochoa, ER ;
Vacanti, JP ;
Fauza, DO .
JOURNAL OF PEDIATRIC SURGERY, 2002, 37 (12) :1720-1725
[8]
Fetal tissue engineering: In utero tracheal augmentation in an ovine model [J].
Fuchs, JR ;
Terada, S ;
Ochoa, ER ;
Vacanti, JP ;
Fauza, DO .
JOURNAL OF PEDIATRIC SURGERY, 2002, 37 (07) :1000-1006
[9]
The history and current status of tissue engineering: The future of pediatric surgery [J].
Grikscheit, TC ;
Vacanti, JP .
JOURNAL OF PEDIATRIC SURGERY, 2002, 37 (03) :277-288
[10]
Kim BS, 2000, WORLD J UROL, V18, P2, DOI 10.1007/s003450050002