Separation of the branch chain hexose, hamamelose, by high-performance liquid chromatography, and amperometric detection of hamamelose and related compounds

被引：2

作者：

Andralojc, PJ

Keys, AJ

Adam, AL

Parry, MAJ ^{[1
]}

机构：

[1] IACR Rothamsted, Dept Physiol & Biochem, Harpenden AL5 2JQ, Herts, England

[2] Dionex UK Ltd, Surrey GU15 2XA, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 814卷 / 1-2期

关键词：

plant materials; sugars; hamamelose; carboxyarabinitol; rubisco; enzymes;

D O I：

10.1016/S0021-9673(98)00409-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The identification and purification of the branch chain hexose hamamelose (2-hydroxymethyl-D-ribose) from plant leaves is described. The procedure includes metabolite extraction in acid and a series of isocratic HPLC separations. Amperometric detection of hamamelose is shown to be quantitative, having a detection limit below 1 nmol. Similar detection of the related compound, 2-carboxy-D-arabinitol, and its monophosphate ester, yields unusually small peaks. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：105 / 110

页数：6

共 12 条

[1] Conversion of D-hamamelose into 2-carboxy-D-arabinitol and 2-carboxy-D-arabinitol 1-phosphate in leaves of Phaseolus vulgaris L. [J].