E2F transcription factor-1 regulates oxidative metabolism

被引:218
作者
Blanchet, Emilie [1 ,2 ,3 ,4 ,5 ,6 ]
Annicotte, Jean-Sebastien [1 ,2 ,3 ,4 ,5 ,6 ]
Lagarrigue, Sylviane [1 ,2 ,3 ,4 ,5 ,6 ]
Aguilar, Victor [1 ,2 ,3 ,4 ]
Clape, Cyrielle [1 ,2 ,3 ,4 ]
Chavey, Carine [1 ,2 ,3 ,4 ,5 ,6 ]
Fritz, Vanessa [1 ,2 ,3 ,4 ,5 ,6 ]
Casas, Francois [7 ]
Apparailly, Florence [8 ]
Auwerx, Johan [9 ]
Fajas, Lluis [1 ,2 ,3 ,4 ,5 ,6 ]
机构
[1] Inst Rech Cancerol Montpellier, IRCM, F-34298 Montpellier, France
[2] INSERM, U896, F-34298 Montpellier, France
[3] Univ Montpellier 1, F-34298 Montpellier, France
[4] CRLC Val dAurelle Paul Lamarque, F-34298 Montpellier, France
[5] Inst Genet Mol Montpellier, IGMM, F-34293 Montpellier, France
[6] CNRS, UMR5535, F-34293 Montpellier, France
[7] INRA, UMR0866, F-34060 Montpellier, France
[8] INSERM, U844, F-34295 Montpellier, France
[9] Ecole Polytech Fed Lausanne, CH-1115 Lausanne, Switzerland
基金
美国国家卫生研究院; 瑞士国家科学基金会; 欧洲研究理事会;
关键词
ACTIVATED-RECEPTOR-GAMMA; SKELETAL-MUSCLE; ADIPOCYTE DIFFERENTIATION; GENE-EXPRESSION; ENZYME-ACTIVITY; PGC-1-ALPHA; GROWTH; COACTIVATOR; REPRESSION; PROTECTS;
D O I
10.1038/ncb2309
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cells respond to stress by coordinating proliferative and metabolic pathways. Starvation restricts cell proliferative (glycolytic) and activates energy productive (oxidative) pathways. Conversely, cell growth and proliferation require increased glycolytic and decreased oxidative metabolism levels(1). E2F transcription factors regulate both proliferative and metabolic genes(2,3). E2Fs have been implicated in the G1/S cell-cycle transition, DNA repair, apoptosis, development and differentiation(2-4). In pancreatic beta-cells, E2F1 gene regulation facilitated glucose-stimulated insulin secretion(5,6). Moreover, mice lacking E2F1 (E2f1(-/-)) were resistant to diet-induced obesity(4). Here, we show that E2F1 coordinates cellular responses by acting as a regulatory switch between cell proliferation and metabolism. In basal conditions, E2F1 repressed key genes that regulate energy homeostasis and mitochondrial functions in muscle and brown adipose tissue. Consequently, E2f1(-/-) mice had a marked oxidative phenotype. An association between E2F1 and pRB was required for repression of genes implicated in oxidative metabolism. This repression was alleviated in a constitutively active CDK4 (CDK4(R24C)) mouse model or when adaptation to energy demand was required. Thus, E2F1 represents a metabolic switch from oxidative to glycolytic metabolism that responds to stressful conditions.
引用
收藏
页码:1146 / U184
页数:9
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