Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site

被引：179

作者：

Josephson, K

Logsdon, NJ

Walter, MR ^{[1
]}

机构：

[1] Univ Alabama Birmingham, Ctr Macromol Crystallog, Birmingham, AL 35294 USA

[2] Univ Alabama Birmingham, Dept Microbiol, Birmingham, AL 35294 USA

来源：

IMMUNITY | 2001年 / 15卷 / 01期

关键词：

D O I：

10.1016/S1074-7613(01)00169-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Interleukin 10 (IL-10) is a dimeric cytokine that plays a central role in suppressing inflammatory responses. These activities are dependent on the interaction of IL-10 with its high-affinity receptor (IL-10R1). This intermediate complex must subsequently recruit the low-affinity IL-10R2 chain before cell signaling can occur. Here we report the 2.9 Angstrom crystal structure of IL-10 bound to a soluble form of IL-10R1 (sIL-10R1). The complex consists of two IL-10s and four sIL-10R1 molecules. Several residues in the IL-10/sIL-10R1 interface are conserved in all IL-10 homologs and their receptors. The data suggests that formation of the active IL-10 signaling complex occurs by a novel molecular recognition paradigm where IL-10R1 and IL-10R2 both recognize the same binding site on IL-10.

引用

页码：35 / 46

页数：12

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