Focusing astigmatic Gaussian beams through optical systems with a high numerical aperture

被引：18

作者：

Gregor, I ^{[1
]}

Enderlein, J ^{[1
]}

机构：

[1] Forschungszentrum Julich, Inst Biol Informat Proc 1 & 2, D-52425 Julich, Germany

来源：

OPTICS LETTERS | 2005年 / 30卷 / 19期

关键词：

D O I：

10.1364/OL.30.002527

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We theoretically derive the electric field distribution of an astigmatic Gaussian laser beam after it is focused through a high-aperture objective. We show that astigmatism values that are hard to detect in the collimated laser beam can have a large effect after diffraction-limited focusing. Such astigmatic beams may be frequently encountered in fluorescence correlation measurements and in laser-scanning confocal microscopy. We present experimental measurements of the excitation intensity distribution measured by 3D scanning of single fluorescent molecules immobilized on a glass surface. (c) 2005 Optical Society of America.

引用

页码：2527 / 2529

页数：3

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