The Effect of Different Concentration of Epibrassinolide on Chlorophyll, Protein and Anthocyanin Content and Peroxidase Activity in Excised Red Cabbage (Brassica Oleraceae L.) Cotyledons

被引:9
作者
Cag, S. [1 ]
Goren-Saglam, N. [1 ]
Cingil-Baris, C. [2 ]
Kaplan, E. [1 ]
机构
[1] Istanbul Univ, Fac Sci, Dept Bot, Istanbul, Turkey
[2] Istanbul Univ, Fac Educ, Dept Primary Educ, Istanbul, Turkey
关键词
epibrassinolid; cotyledon growth; chlorophyll; protein; anthocyanin; peroxidase activity;
D O I
10.1080/13102818.2007.10817487
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Brassinosteroids are steroidal plant hormones that influence varied growth and development processes such as germination of seeds, flowering, senescence and abscission. This study aimed to explore the effect of cotyledon growth by different epibrassinolid (epiBL) concentrations in excised red cabbage cotyledons. As an experimental material, the cotyledons of 8-day-old Brassica oleraceae seedlings, excised from the petiole were incubated in 3 nil distilled water (control group) and in epiBL of various concentrations (0. 001, 0. 1 and 10 mu M) for 3 days. Following incubation, peroxidase activity, total chlorophyll, soluble protein and anthocyanin contents were determined. The data indicate that the effect of epiBL on the growth of excised red cabbage cotyledons show difference related to concentration.
引用
收藏
页码:422 / 425
页数:4
相关论文
共 37 条
[1]   COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].
ARNON, DI .
PLANT PHYSIOLOGY, 1949, 24 (01) :1-15
[3]   COMPARATIVE STUDIES ON TOBACCO PITH AND SWEET POTATO ROOT ISOPEROXIDASES IN RELATION TO INJURY, INDOLEACETIC-ACID, AND ETHYLENE EFFECTS [J].
BIRECKA, H ;
BRIBER, KA ;
CATALFAMO, JL .
PLANT PHYSIOLOGY, 1973, 52 (01) :43-49
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]  
BRAUN P, 1984, P 6 C PHOT, P461
[6]   Biological effects of brassinosteroids [J].
Brosa, C .
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 34 (05) :339-358
[7]   Comparison of the effects of meta-topolin and other cytokinins on chlorophyll and protein contents and peroxidase activity in cucumber cotyledons [J].
Çag, S ;
Palavan-Ünsal, N ;
Büyüktunçer, D .
ISRAEL JOURNAL OF PLANT SCIENCES, 2003, 51 (04) :261-265
[8]  
CINGIL C, 2005, THESIS ISTANBUL U
[9]  
Clouse S. D., 1991, Brassinosteroids. Chemistry, bioactivity and applications., P122
[10]   EFFECT OF BRASSINOLIDE ON GENE-EXPRESSION IN ELONGATING SOYBEAN EPICOTYLS [J].
CLOUSE, SD ;
ZUREK, DM ;
MCMORRIS, TC ;
BAKER, ME .
PLANT PHYSIOLOGY, 1992, 100 (03) :1377-1383