Conditional control of gene expression in the mouse

被引：544

作者：

Lewandoski, M ^{[1
]}

机构：

[1] NCI, Frederick Canc Res & Dev Ctr, Sect Genet Vertebrate Dev, Lab Canc & Dev Biol, Frederick, MD 21702 USA

来源：

NATURE REVIEWS GENETICS | 2001年 / 2卷 / 10期

关键词：

D O I：

10.1038/35093537

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

One of the most powerful tools that the molecular biology revolution has given us is the ability to turn genes on and off at our discretion. In the mouse, this has been accomplished by using binary systems in which gene expression is dependent on the interaction of two components, resulting in either transcriptional transactivation or DNA recombination. During recent years, these systems have been used to analyse complex and multi-staged biological processes, such as embryogenesis and cancer, with unprecedented precision. Here, I review these systems and discuss certain studies that exemplify the advantages and limitations of each system.

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页码：743 / 755

页数：13

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