Transgenic Indica rice breeding line IR58 expressing a synthetic cryIA(b) gene from Bacillus thuringiensis provides effective insect pest control

被引：145

作者：

Wunn, J ^{[1
]}

Kloti, A ^{[1
]}

Burkhardt, PK ^{[1
]}

Biswas, GCG ^{[1
]}

Launis, K ^{[1
]}

Iglesias, VA ^{[1
]}

Potrykus, I ^{[1
]}

机构：

[1] CIBA GEIGY CORP,RES TRIANGLE PK,NC 27709

来源：

BIO-TECHNOLOGY | 1996年 / 14卷 / 02期

关键词：

D O I：

10.1038/nbt0296-171

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Indica rice breeding line IR58 was transformed by particle bombardment with a truncated version of a synthetic cryIA(b) gene from Bacillus thuringiensis. This gene is expressed under control of the CaMV 35S promoter and allows efficient production of the lepidopteran specific delta-endotoxin. R(0), R(1) and R(2) generation plants displayed a significant insecticidal effect on several lepidopterous insect pests. Feeding studies showed mortality rates of up to 100% for two of the most destructive insect pests of rice in Asia, the yellow stem borer (Scirpophaga incertulas) and the striped stem borer (Chile suppressalis), and feeding inhibition of the two leaffolder species Cnaphalocrocis medinalis and Marasmia patnalis. Introduction of stem borer resistance into the germplasm of an Indica rice breeding line now makes this agronomically important trait available for conventional rice breeding programs.

引用

页码：171 / 176

页数：6

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