Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers

被引：47

作者：

Masojc, P ^{[1
]}

Mysków, B ^{[1
]}

Milczarski, P ^{[1
]}

机构：

[1] Acad Agr Szczecin, Dept Plant Breeding, PL-71434 Szczecin, Poland

来源：

THEORETICAL AND APPLIED GENETICS | 2001年 / 102卷 / 08期

关键词：

genetic map; Secale cereale L; RAPD; isozymes; RFLP;

D O I：

10.1007/s001220000512

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

RFLP-based genetic map of rye, developed previously using a cross of lines DS2xRXL10 (F-2 generation), was extended with 69 RAPD and 12 isozyme markers. The actual map contains 282 markers dispersed on all seven chromosomes and spans a distance of 1,140 cM. The efficiency of mapping RAPD markers was close to ten loci per 100-screened arbitrary primers. A strong selection of polymorphic, intensive and reproducible fragments was necessary to reveal individual marker loci that could be assigned to rye chromosomes. Newly mapped markers cover a substantial part of the rye genome and constitute a valuable tool suitable for map saturation, marker-aided selection and phenetic studies. A specific nomenclature for the RAPD loci mapped on individual rye chromosomes, which could be helpful in managing of accumulating data, is proposed.

引用

页码：1273 / 1279

页数：7

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