Lysozyme inactivation by inert gas bubbling:: kinetics in a bubble column reactor

被引：33

作者：

Caussette, M ^{[1
]}

Gaunand, A ^{[1
]}

Planche, H ^{[1
]}

Colombié, S ^{[1
]}

Monsan, P ^{[1
]}

Lindet, B ^{[1
]}

机构：

[1] Ecole Natl Super Mines, Biotechnol Lab, F-75006 Paris, France

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1999年 / 24卷 / 07期

关键词：

lysozyme; gas; inactivation; interface; bubble column; kinetics;

D O I：

10.1016/S0141-0229(98)00151-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This article focuses on lysozyme inactivation at gas-liquid interfaces. Bubbling nitrogen in a lysozyme aqueous solution strongly enhances enzyme inactivation. At a 150-ml min(-1) flow rate, the half-life of lysozyme is 12 min while in the same conditions without bubbling no activity loss is observed after 8 h. Reported effects vary linearly with the interfacial area which shows that the inactivation is an interfacial mechanism. The inactivation induced by nitrogen bubbling strongly depends on temperature and pH acting on the adsorption process. This stud points out on the one hand the importance of controlling gas-liquid interfaces in bioreactors and on the other hand the potentialities of an inactivation process using gas-liquid interfaces. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：412 / 418

页数：7

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