Magnetization Transfer Helps Detect Intestinal Fibrosis in an Animal Model of Crohn Disease

被引：141

作者：

Adler, Jeremy ^{[1
]}

Swanson, Scott D. ^{[1
]}

Schmiedlin-Ren, Phyllissa ^{[1
]}

Higgins, Peter D. R. ^{[1
]}

Golembeski, Christopher P. ^{[1
]}

Polydorides, Alexandros D. ^{[1
]}

McKenna, Barbara J. ^{[1
]}

Hussain, Hero K. ^{[1
]}

Verrot, Trevor M. ^{[1
]}

Zimmermann, Ellen M. ^{[1
]}

机构：

[1] Univ Michigan, Med Ctr, Dept Pediat, Div Gastroenterol, Ann Arbor, MI 48109 USA

来源：

RADIOLOGY | 2011年 / 259卷 / 01期

基金：

美国国家卫生研究院;

关键词：

INFLAMMATORY-BOWEL-DISEASE; GRANULOMATOUS ENTEROCOLITIS; RATS; ENTEROGRAPHY; MANAGEMENT; ARTHRITIS; CONTRAST; FEATURES; IMAGES;

D O I：

10.1148/radiol.10091648

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

100231 [临床病理学]; 100902 [航空航天医学];

摘要：

Purpose: To determine the utility of magnetization transfer (MT) in the identification and quantification of intestinal fibrosis in a rat model of Crohn disease. Materials and Methods: The university committee on the use and care of animals approved this study (UCUCA 08592). Lewis rats injected subserosally with peptidoglycan-polysaccharide (PG-PS) develop bowel inflammation 1 day after laparotomy (early phase) and fibrosis starting 14 days after laparotomy (late phase). The authors performed 2.0-T magnetic resonance (MR) imaging in 25 rats injected with PG-PS and 13 injected with human serum albumin (HSA) (control animals). Imaging was performed before laparotomy and on a weekly basis thereafter for up to 28 days. The MT ratio in the bowel wall was calculated. Resected cecal tissue was scored for inflammation and fibrosis. Tissue fibrosis was determined with colorimetric analysis of trichrome-stained specimens. Collagen content was measured with Western blot analysis. Statistical analyses were performed with the Student t test for continuous bivariate comparisons, the Pearson correlation for continuous variables, and the Spearman correlation for ordinal variables. Results: All rats developed early inflammation, which subsided over time. Rats injected with PG-PS developed increased fibrosis in the late phase, whereas control rats did not. The mean MT ratio of rats injected with PG-PS with late-phase fibrosis was higher than that in rats with early phase inflammation (P = .017). In addition, the MT ratio of rats injected with PG-PS with late-phase fibrosis was higher than that of control animals that did not develop fibrosis in the late phase (P = .0001). The MT ratio of control animals remained unchanged over time as inflammation subsided. The MT ratio in rats injected with PG-PS showed correlation with tissue fibrosis (r = 0.63). The MT ratio showed correlation with tissue collagen (R = 0.74). The positive and negative predictive values of the MT ratio in the prediction of fibrosis were 92% (12 of 13 rats) and 83% (five of six rats), respectively. Conclusion: These results indicate that MT is sensitive to bowel wall fibrosis as occurs in Crohn strictures. (C) RSNA, 2011

引用

页码：127 / 135

页数：9

共 30 条

[1]

Crohn disease: Mural attenuation and thickness at contrast-enhanced CT enterography - Correlation with endoscopic and histologic findings of inflammation [J].