Glucagon-like peptide-1 protects against cardiac microvascular endothelial cells injured by high glucose

被引：25

作者：

Ge, Guang-Hao ^{[1
,2
]}

Dou, Hong-Jie ^{[3
,4
]}

Yang, Shuan-Suo ^{[1
,2
]}

Ma, Jiang-Wei ^{[1
,2
]}

Cheng, Wen-Bo ^{[1
,2
]}

Qiao, Zeng-Yong ^{[1
,2
]}

Hou, Yue-Mei ^{[1
,2
]}

Fang, Wei-Yi ^{[5
]}

机构：

[1] Fangxian Dist Cent Hosp, Dept Cardiol, Shanghai 201499, Peoples R China

[2] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp South Campus 6, Dept Cardiol, Shanghai 201499, Peoples R China

[3] Fengxian Dist Cent Hosp, Dept Intens Care Unit, Shanghai 201499, Peoples R China

[4] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp South Campus 6, Dept Cardiol, Dept Intens Care Unit, Shanghai 201499, Peoples R China

[5] Shanghai Jiao Tong Univ, Affiliated Chest Hosp, Dept Cardiol, Shanghai 200052, Peoples R China

来源：

ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2015年 / 8卷 / 01期

关键词：

Glucagon-like peptid-1; Cardiac microvascular endothelial cell; ROS; Rho/ROCK; OXIDATIVE STRESS; LIRAGLUTIDE PROTECTS; GLP-1; ANALOG; DISEASE; KINASE; COMPLICATIONS; ACTIVATION; PREVENTION; DAMAGE; RISK;

D O I：

10.1016/S1995-7645(14)60191-7

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

100235 [预防医学];

摘要：

Objective: To investigate the protective effect of glucagon-like peptid-1 (GLP-1) against cardiac microvascular endothelial cell (CMECs) injured by high glucose. Methods: CMECs were isolated and cultured. Superoxide assay kit and dihydroethidine (DHE) staining were used to assess oxidative stress. TUNEL, staining and caspase 3 expression were used to assess the apoptosis of CMECs. H89 was used to inhibit cAMP/PKA pathway; fasudil was used to inhibit Rho/ROCK pathway. The protein expressions of Rho, ROCK were examined by Western blot analysis. Results: High glucose increased the production of ROS, the activity of NADPH, the apoptosis rate and the expression level of Rho/ROCK in CMECs, while GLP-1 decreased high glucose induced ROS production, the NADPH activity and the apoptosis rate and the expression level of Rho/ ROCK in CMECs, the difference were statistically significant (P<0.05). Conclusions: GLP-1 could protect the cardiac microvessels against oxidative stress and apoptosis. The protective effects of GLP-1 are dependent on downstream inhibition of Rho through a cAMP/PKA-dependent manner, resulting in a subsequent decrease in the expression of NADPH oxidase.

引用

页码：73 / 78

页数：6

共 29 条

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Detection of myocardial microvascular disease using contrast echocardiography during adenosine stress in type 2 diabetes mellitus: Prospective comparison with single-photon emission computed tomography [J].