Multi-metal binding site of serum albumin

被引：266

作者：

Bal, W

Christodoulou, J

Sadler, PJ

Tucker, A

机构：

[1] Univ Wroclaw, Fac Chem, PL-50383 Wroclaw, Poland

[2] Univ London, Birkbeck Coll, Biomed NMR Ctr, London, England

[3] Univ Edinburgh, Dept Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1998年 / 70卷 / 01期

关键词：

serum albumin; copper(II) albumin complexes; nickel(II) albumin complexes; affinity constants; circular dichroism;

D O I：

10.1016/S0162-0134(98)00010-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular dichroism and electron spin resonance spectroscopy are used to investigate the second specific metal binding site on human, bovine and porcine albumins. Ni(II), Zn(II) and Cd(II) can displace Cu(II) from the second Cu(II) site but not from the first strong site of human and bovine albumins (the N-terminal site). The second Cu(II) binds more strongly than the other metal ions to the second site of all three proteins, except Zn(II) binding to porcine albumin which is ca. 10 x stronger than Cu(II). The second Cu(II) site appears to be a tetragonal {2N,4O} site. (C) 1998 Elsevier Science Inc. All rights reserved.

引用

页码：33 / 39

页数：7

共 42 条

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