DNA polymerase λ mediates a back-up base excision repair activity in extracts of mouse embryonic fibroblasts

被引：127

作者：

Braithwaite, EK ^{[1
]}

Prasad, R ^{[1
]}

Shock, DD ^{[1
]}

Hou, EW ^{[1
]}

Beard, WA ^{[1
]}

Wilson, SH ^{[1
]}

机构：

[1] NIEHS, Struct Biol Lab, NIH, Res Triangle Pk, NC 27709 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2005年 / 280卷 / 18期

关键词：

D O I：

10.1074/jbc.M411864200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian DNA polymerase (pol) lambda is a member of the X-family of DNA polymerases and has striking enzymatic and structural similarities to mammalian DNA pol beta. Because pol beta provides two important enzymatic activities for base excision repair (BER), we examined whether pol lambda might also contribute to BER. We used extracts from mouse embryonic fibroblasts representing wild-type and null genotypes for pol beta and pol lambda. In combination with neutralizing antibodies against pol beta and pol lambda, our results show a BER deficiency in the pol lambda -/- cell extract compared with extract from isogenic wild-type cells. In addition, the pol lambda antibody strongly reduced in vitro BER in the pol beta -/- cell extract. These data indicate that pol lambda is able to contribute to BER in mouse fibroblast cell extract.

引用

页码：18469 / 18475

页数：7

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