Characterization of chitosan and chitosan-glucan complex extracted from the cell wall of fungus Gongronella butleri USDB 0201 by enzymatic method

被引：30

作者：

Nwe, Nitar ^{[1
,2
,3
,4
]}

Stevens, Willem F. ^{[4
,5
]}

Tokura, Seiichi ^{[1
,2
]}

Tamura, Hiroshi ^{[1
,2
]}

机构：

[1] Kansai Univ, Fac Chem Mat & Bioengn, Osaka 5648680, Japan

[2] Kansai Univ, HRC, Osaka 5648680, Japan

[3] Japan Soc Promot Sci, Tokyo, Japan

[4] Asian Inst Technol, Bangkok 12120, Thailand

[5] Mahidol Univ, Fac Sci, Ctr Excellence Shrimp Biotechnol, Bangkok 10400, Thailand

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2008年 / 42卷 / 03期

基金：

日本学术振兴会;

关键词：

chitin/chitosan; chitosan - glucan complex (CGC); fungal cell wall; glucan; glycosidic bond;

D O I：

10.1016/j.enzmictec.2007.10.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The fungus Gongronella butleri USDB 0201 was grown on sweet potato pieces supplemented with mineral and urea solution. Mycelia were harvested after 7 days of cultivation. Chitosan in the fungal cell wall exists in two forms, free chitosan and chitosan bounded to beta-glucan. The linkage between chitosan and beta-glucan in the chitosan-glucan complex (CGC) was successfully cleaved using a heat stable alpha-amylase. The resultant chitosan and beta-glucan polymeric moieties were characterized. Data from elementary analysis, IR and C-13 NMR spectroscopy confirmed that all intramolecular glycosidic linkages in either chitosan or glucan polymeric moieties are in the beta-configuration. Data from specific enzymatic degradation of the CGC obtained from different treatments and reducing sugar analysis indicate that chitosan and glucan are linked by an intermolecular alpha-1,4 glycosidic bond. These evidences supported to prove the beta-linked chitosan and P-linked glucan are linked with alpha-1,4 glycosidic bond. However heat stable alpha-amylase enzyme could not cleave the CGC completely. Therefore high yield with pure chitosan could be obtained from the cell wall of fungus G. butleri USDB 0201 by Termamyl enzyme treatment. (c) 2007 Elsevier Inc. All fights reserved.

引用

页码：242 / 251

页数：10

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