Determinants of RNA Quality from FFPE Samples

被引：309

作者：

von Ahlfen, Silke ^{[1
]}

Missel, Andreas ^{[1
]}

Bendrat, Klaus

Schlumpberger, Martin ^{[1
]}

机构：

[1] QIAGEN GmbH, Hilden, Germany

来源：

PLOS ONE | 2007年 / 2卷 / 12期

关键词：

D O I：

10.1371/journal.pone.0001261

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The large archives of formalin-fixed paraffin-embedded (FFPE) tissue specimens that exist are a highly valuable source of sample material for molecular biological analysis, including gene expression profiling. However, current data on adverse effects of standard pathological practice on the usefulness of biomolecular analytes obtained from such archived specimens is largely anecdotal. Here, we present a systematic examination of the most relevant parameters for integrity and useability of RNA obtained from FFPE samples, including storage time and conditions, fixation time, and specimen size. The results are particularly relevant for any application relying on cDNA synthesis as an initial step of the procedure, such as RT-PCR, and microarray analysis.

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