Fluorescence resonance energy transfer dye nucleotide terminators: A new synthetic approach for high-throughout dna sequencing

被引：4

作者：

Nampalli, S ^{[1
]}

Khot, M ^{[1
]}

Nelson, JR ^{[1
]}

Flick, PK ^{[1
]}

Fuller, CW ^{[1
]}

Kumar, S ^{[1
]}

机构：

[1] Amersham Pharmacia Biotech, Piscataway, NJ 08855 USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002307

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase(TM) II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.

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页码：361 / 367

页数：7

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