Genomic exploration of the hemiascomycetous yeasts: 2. Data generation and processing

被引：41

作者：

Artiguenave, F ^{[1
]}

Wincker, P ^{[1
]}

Brottier, P ^{[1
]}

Duprat, S ^{[1
]}

Jovelin, F ^{[1
]}

Scarpelli, C ^{[1
]}

Verdier, J ^{[1
]}

Vico, V ^{[1
]}

Weissenbach, J ^{[1
]}

Saurin, W ^{[1
]}

机构：

[1] GENOSCOPE, Ctr Natl Sequencage, Evry, France

来源：

FEBS LETTERS | 2000年 / 487卷 / 01期

关键词：

high-throughput sequencing; sequence analysis; genome; hemiascomycete;

D O I：

10.1016/S0014-5793(00)02273-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE, These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：13 / 16

页数：4

共 9 条

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