Cytogenetic characterization of the human prostate cancer cell line P69SV40T and its novel tumorigenic sublines M2182 and M15

被引:44
作者
JacksonCook, C
Bae, V
Edelman, W
Brothman, A
Ware, J
机构
[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PATHOL,RICHMOND,VA 23298
[2] UNIV UTAH,DEPT PEDIAT,SALT LAKE CITY,UT
[3] UNIV UTAH,DEPT HUMAN GENET,SALT LAKE CITY,UT
关键词
D O I
10.1016/0165-4608(95)00232-4
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Cytogenetic studies of a SV40T antigen immortalized human prostate epithelial cell line (P69SV40T) and its increasingly tumorigenic tumor sublines, designated M2182 and M15, were done with GTG-banding and multicolor fluorescence in situ hybridization (FISH). The parental line and each of the two sublines were near-diploid and contained several consistent abnormalities. Two structural chromosome anomalies were noted in all three lines; a der(7)t(5;20;7) and a der(5)t(5;9). Abnormalities that were acquired and retained in the tumor sublines after in vivo and/or in vitro selection included a der(1)t(1;8), der(3)t(3;14), der(20)t(7;20), and der(X)t(X;11). Findings unique to subline M2182 were a der(11)t(5;11) and -14. Those unique to M15 were a der(16)t(16;19) and -Y. Chromosome imbalances resulting from numerical and/or structural abnormalities in the tumor sublines involved several chromosome regions that have previously been implicated in human prostate cancer; such as loss of Xp, Y, 3p (M2182 and M15), 16q (M15), and gains for 5q (M2182) and 8q (M2182 and M15). Collectively, the characterization of these lines should assist with the localization of chromosome regions, and possibly genes, that are important in the development and progression of human prostate cancer.
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页码:14 / 23
页数:10
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