Rational design of "Turn-On" allosteric DNAzyme catalytic beacons for aqueous mercury ions with ultrahigh sensitivity and selectivity

被引：531

作者：

Liu, Juewen ^{[1
]}

Lu, Yi ^{[1
]}

机构：

[1] Univ Illinois, Beckman Inst Adv Sci & Technol, Dept Chem, Urbana, IL 61801 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 40期

关键词：

DNA; enzymes; fluorescence; mercury; sensors;

D O I：

10.1002/anie.200702006

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

© 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. (Figure Presented) The mercury is rising: A DNAzyme with several thymine residues located close to the catalytic site is rendered inactive. However, thymine-Hg2+-thymine interactions transform the DNAzyme into an active enzyme. When fluorophore/quencher pairs are attached, the DNAzyme can be converted into a highly sensitive and selective mercury sensor with a detection limit of 2.4 nM and a greater than 100 000-fold selectivity over other metal ions.

引用

页码：7587 / 7590

页数：4

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