Synthesis of the Streptomyces lividans maltodextrin ABC transporter depends on the presence of the regulator MalR

被引：19

作者：

Schlösser, A ^{[1
]}

Weber, A ^{[1
]}

Schrempf, H ^{[1
]}

机构：

[1] Univ Osnabruck, Fachbereich Biol Chem, D-49069 Osnabruck, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 196卷 / 01期

关键词：

ABC transporter; maltose/maltodextrin; MalR repressor; Reg1; repressor; regulation of transcription; glucose repression; Streptomyces;

D O I：

10.1016/S0378-1097(00)00566-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During growth with maltotriose or amylose, Streptomyces lividans and Streptomyces coelicolor A3(2) synthesize a maltodextrin uptake system with highest specificity for maltotriose. The transport activity is absent in mutants of S. coelicolor A3(2) lacking a functional MalE binding protein. Cloning and sequencing data suggest that the mal operon of S. coelicolor A3(2) corresponds to the one of S. lividans and that the deduced S. lividans Reg1 amino acid sequence is identical to that of MalR from S. coelicolor A3(2). It can be concluded that both strains have the same ABC transport system for maltodextrins. The S. lividans malR was cloned in Escherichia coli in frame with six histidine-encoding codons. The resulting, purified 6HisMalR(Sl) was shown to bind to two motifs within the S. lividans malR-malE intergenic region and to dissociate in the presence of maltopentaose. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：77 / 83

页数：7

共 23 条

[1]

Ausubel FM, 1995, SHORT PROTOCOLS MOL

[2]

Boos W., 1996, Escherichia coli and Salmonella, V1, P1175

[3] MALTOSE AND MALTOTRIOSE CAN BE FORMED ENDOGENOUSLY IN ESCHERICHIA-COLI FROM GLUCOSE AND GLUCOSE-1-PHOSPHATE INDEPENDENTLY OF ENZYMES OF THE MALTOSE SYSTEM [J].

DECKER, K ;

PEIST, R ;

REIDL, J ;

KOSSMANN, M ;

BRAND, B ;

BOOS, W .

JOURNAL OF BACTERIOLOGY, 1993, 175 (17) :5655-5665

[4] Catabolite repression mediated by the catabolite control protein CcpA in Staphylococcus xylosus [J].

Egeter, O ;

Bruckner, R .

MOLECULAR MICROBIOLOGY, 1996, 21 (04) :739-749

[5] IDENTIFICATION OF ENDOGENOUS INDUCERS OF THE MAL REGULON IN ESCHERICHIA-COLI [J].

EHRMANN, M ;

BOOS, W .

JOURNAL OF BACTERIOLOGY, 1987, 169 (08) :3539-3545

[6] Molecular analysis of the malR gene of Clostridium butyricum NCIMB 7423, a member of the LacI-GalR family of repressor proteins [J].

Goda, SK ;

Eisa, O ;

Akhter, M ;

Minton, NP .

FEMS MICROBIOLOGY LETTERS, 1998, 165 (01) :193-200

[7] Biochemical identification of a lipoprotein with maltose-binding activity in the thermoacidophilic Gram-positive bacterium Alicyclobacillus acidocaldarius [J].

Herrmann, A ;

Schlosser, A ;

Schmid, R ;

Schneider, E .

RESEARCH IN MICROBIOLOGY, 1996, 147 (09) :733-737

[8] A CELLULASE XYLANASE-NEGATIVE MUTANT OF STREPTOMYCES-LIVIDANS-1326 DEFECTIVE IN CELLOBIOSE AND XYLOBIOSE UPTAKE IS MUTATED IN A GENE ENCODING A PROTEIN HOMOLOGOUS TO ATP-BINDING PROTEINS [J].

HURTUBISE, Y ;

SHARECK, F ;

KLUEPFEL, D ;

MOROSOLI, R .

MOLECULAR MICROBIOLOGY, 1995, 17 (02) :367-377

[9] High-affinity maltose binding and transport by the thermophilic anaerobe Thermoanaerobacter ethanolicus 39E [J].

Jones, CR ;

Ray, M ;

Dawson, KA ;

Strobel, HJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (03) :995-1000

[10] Crystal structure of the lactose operon repressor and its complexes with DNA and inducer [J].

Lewis, M ;

Chang, G ;

Horton, NC ;

Kercher, MA ;

Pace, HC ;

Schumacher, MA ;

Brennan, RG ;

Lu, PZ .

SCIENCE, 1996, 271 (5253) :1247-1254

← 1 2 3 →