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Synthesis of luciferyl coenzyme A: A bioluminescent substrate for firefly luciferase in the presence of AMP

被引：10
作者：
Fraga, H
Fontes, R
da Silva, JCGE
机构：
[1] Univ Porto, Fac Ciencias, Dept Chem, LAQUIPAI, P-4169007 Oporto, Portugal
[2] Univ Porto, Fac Med, Biochem Dept U38FCT, P-4200319 Oporto, Portugal
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 22期
关键词：
adenosine monophosphate; bioluminescence enzymes; firefly luciferase; total synthesis;
D O I：
10.1002/anie.200462934
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Chemical Equation Presented) Shedding light on luciferase: Luciferyl coenzyme A (1) was synthesized and it was found that luciferase catalyzes light production from luciferyl coenzyme A and AMP. The results of this study open perspectives for new bioanalytical methods and are strong evidence for the evolutionary relationship between firefly luciferase and acyl-CoA synthetases. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3427 / 3429
页数：3
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