Pathogen specific carbohydrate antigen microarrays:: a chip for detection of Salmonella O-antigen specific antibodies

被引：55

作者：

Blixt, Ola ^{[1
]}

Hoffmann, Julia ^{[1
]}

Svenson, Stefan ^{[2
]}

Norberg, Thomas ^{[3
]}

机构：

[1] Scripps Res Inst, Dept Mol Biol, Glycan Array Synth Core D, Consortium Funct Glycom, La Jolla, CA 92037 USA

[2] Swedish Inst Infect Dis Control, S-17182 Solna, Sweden

[3] Uppsala Univ, Dept Biochem & Organ Chem, S-75123 Uppsala, Sweden

来源：

GLYCOCONJUGATE JOURNAL | 2008年 / 25卷 / 01期

关键词：

glycanarray; polysaccharide; oligosaccharide; antibody; salmonellosis;

D O I：

10.1007/s10719-007-9045-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Salmonella O-antigen microarray was developed by covalent coupling of oligosaccharide antigens specific for serogroups Salmonella enterica sv. Paratyphi (group A), Typhimurium (group B) and Enteritidis (group D). Antibodies were correctly detected in sera from patients with culture verified salmonellosis. High serogroup-specificity was seen with the disaccharide antigens. With the larger antigens, containing the backbone sequence Man alpha 1-2Rha alpha 1-2Gal (MRG), common backbone-specific antibodies (O-antigen 12) were also detected. This is "proof of principle" that pathogen-specific carbohydrate antigen microarrays constitute a novel technology for rapid and specific serological diagnosis in either individual patients or larger sero-epidemiological and vaccine studies.

引用

页码：27 / 36

页数：10

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