Combined profile of androgen glucuro- and sulfoconjugates in postcompetition urine of sportsmen: A simple screening procedure using gas chromatography mass spectrometry

被引：60

作者：

Dehennin, L ^{[1
]}

Lafarge, P ^{[1
]}

Dailly, P ^{[1
]}

Bailloux, D ^{[1
]}

Lafarge, JP ^{[1
]}

机构：

[1] CREPS CHATENAY MALABRY,LAB NATL DEPISTAGE DOPAGE,F-92290 CHATENAYMALABRY,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B | 1996年 / 687卷 / 01期

关键词：

androgen; testosterone; epitestosterone;

D O I：

10.1016/S0378-4347(96)00131-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical screening procedure has been developed for the estimation of total androgen conjugates in post-competition urine, using gas chromatography-mass spectrometry with computerized data acquisition and concentration calculation. Rapid acid-catalyzed methanolysis is a key feature of the method, which allows simultaneous cleavage of glucuronides and sulfates. Analytical data generated by this method for testosterone and epitestosterone are in accordance with our previous results obtained by more accurate isotope dilution mass spectrometry. The usefulness of the ratio of testosterone glucuronide-total epitestosterone as an aid for a better discrimination between physiologically high and pharmacologically high ratios of testosterone glucuronide-epitestosterone glucuronide, which was demonstrated previously, has been confirmed here.

引用

页码：85 / 91

页数：7

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