Radiation-induced degradation of DNA bases

被引：5

作者：

Douki, T ^{[1
]}

Delatour, T ^{[1
]}

Martini, R ^{[1
]}

Cadet, J ^{[1
]}

机构：

[1] CEA Grenoble, Lab Les Acides Nucleiques, SCIB, DRFMC, F-38054 Grenoble 9, France

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1999年 / 96卷 / 01期

关键词：

DNA damage; oxidized bases; hydroxyl radical; electron transfer;

D O I：

10.1051/jcp:1999121

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Radio-induced degradation of DNA involves radical processes. A series of lesions among the major bases degradation products has been measured in isolated DNA exposed to gamma radiation in aerated aqueous solution. Degradation can be accounted for by the formation of hydroxyl radicals upon radiolysis of water (indirect effect). The four bases are degraded in high yield. Direct effect has been mimicked by photo-induced electron abstraction from the bases producing their radical cation, Quantification of the modified bases showed that guanine is the preferential target. This can be explained by its lower oxidation potential and charge transfer phenomena.

引用

页码：138 / 142

页数：5

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