Mitochondrial respiratory impairment in streptozotocin-induced diabetic rat heart

被引:43
作者
Tomita, M
Mukae, S
Geshi, E
Umetsu, K
Nakatani, M
Katagiri, T
机构
[1] Third Dept. of Internal Medicine, Showa University, School of Medicine, Tokyo
[2] Third Dept. of Internal Medicine, Showa University, School of Medicine, Shinagawa-ku, Tokyo 142
来源
JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION | 1996年 / 60卷 / 09期
关键词
diabetes mellitus; rat heart; mitochondria; electron transport system; streptozotocin;
D O I
10.1253/jcj.60.673
中图分类号
N09 [自然科学史]; B [哲学、宗教];
学科分类号
01 ; 0101 ; 010108 ; 060207 ; 060305 ; 0712 ;
摘要
The purpose of this study was to examine mitochondrial respiratory impairment in the diabetic heart. Diabetes mellitus was induced in male Wistar rats by intravenous injection of streptozotocin (STZ) for 2 to 16 weeks (Group D). In some of the diabetic rats, insulin was injected for 2 or 3 weeks prior to sacrifice (Group I). Fasting blood glucose was markedly elevated to greater than 300 mg/dl in Group D and was similar to normal glucose levels in Group I. At 2 weeks after STZ injection, state 3 was only 59.1% of that in the control. Complex I and complex V activities were also significantly reduced to 43.4% and 71.7% of those in the control, respectively. These reductions recovered with insulin treatment. This phenomenon persisted for 16 weeks. Morphological studies revealed swelling of the mitochondria and an increase in lipid droplets in diabetic cardiomyocytes, and these were also improved with insulin treatment. We conclude that in the diabetic heart, disturbance of energy production in cardiac mitochondria is generated by the impairment of oxidative phosphorylation due to depression of complex I and complex V. These changes may contribute the cardiac dysfunction that is a complication of diabetes mellitus.
引用
收藏
页码:673 / 682
页数:10
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