Characterization and distribution of amylases during vegetative cell growth and sporulation of Clostridium perfringens

被引:4
作者
Shih, NJ [1 ]
Labbe, RG [1 ]
机构
[1] UNIV MASSACHUSETTS, DEPT FOOD SCI, FOOD MICROBIOL LAB, AMHERST, MA 01003 USA
关键词
Clostridium perfringens; amylase; sporulation;
D O I
10.1139/m96-086
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Clostridium perfringens produced eight extracellular and two intracellular amylolytic activities when examined by zymograms following polyacrylamide gel electrophoresis under native conditions. The major intracellular amylase was isolated from vegetative cells of C. perfringens. It possessed an estimated molecular mass of 112 kDa. Sulfhydryl and phenol functional groups were essential to its activity. The amylase was endo-acting on stanch and also hydrolyzed pullulan. Polyclonal antisera against a purified extracellular amylase did not cross-react with intracellular amylase and the two amylases were biochemically different. The distribution of extracellular amylolytic activities of sporulating cells was different from thar of vegetative cells, whereas the distribution of intracellular amylolytic activities remained identical. A significant increase of a particular amylase (AS) occurred in the extracellular fluid during sporulation compared with that during vegetative growth. Regulation of the excretion of amylase(s) may be sporulation and enterotoxingenicity related.
引用
收藏
页码:628 / 633
页数:6
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