Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques

被引:210
作者
Tamaki, H
Sekiguchi, Y
Hanada, S
Nakamura, K
Nomura, N
Matsumura, M
Kamagata, Y
机构
[1] Natl Inst Adv Ind Sci & Technol, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan
[2] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 305, Japan
关键词
D O I
10.1128/AEM.71.4.2162-2169.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Comparative analysis of bacterial diversity in freshwater sediment collected from a shallow eutrophic lake was performed by using 16S rRNA gene clone library and improved cultivation-based techniques. Our study demonstrated that the use of gellan gum as a gelling reagent instead of agar was more effective at increasing culturability, cultivating a diverse array of novel microbes, and reducing the gaps of the results between molecular and cultivation-based analyses.
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收藏
页码:2162 / 2169
页数:8
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