A nucleic acid sequence-based amplification (NASBA) system for Listeria monocytogenes and simple method for detection of amplimers

被引：1

作者：

Blais, BW ^{[1
]}

Turner, G ^{[1
]}

Sooknanan, R ^{[1
]}

Malek, LT ^{[1
]}

Phillippe, LM ^{[1
]}

机构：

[1] GENESCAPE,MISSISSAUGA,ON L4V 1T4,CANADA

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 03期

关键词：

D O I：

10.1007/BF00158944

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A NASBA system amplifying specific sequences of the Listeria monocytogenes hlyA gene and an immunoenzymatic assay for the detection of amplimers was developed. The immunoenzymatic assay utilized a simple microtiter plate format and an anti-RNA:DNA hybrid antibody for the detection of NASBA product (predominantly RNA) hybridized to an immobilized DNA probe. This highly sensitive isothermal amplification and detection system was reactive with genomic DNA from various L. monocytogenes isolates but not with other Listeria or non-Listeria species.

引用

页码：189 / 194

页数：6

共 10 条

[1] TRANSCRIPTIONAL ENHANCEMENT OF THE LISTERIA-MONOCYTOGENES PCR AND SIMPLE IMMUNOENZYMATIC ASSAY OF THE PRODUCT USING ANTI-RNA - DNA ANTIBODIES [J].