Lighting up the cell surface with evanescent wave microscopy

被引：224

作者：

Toomre, D ^{[1
]}

Manstein, DJ ^{[1
]}

机构：

[1] Max Planck Inst Med Res, Dept Biophys, D-69120 Heidelberg, Germany

来源：

TRENDS IN CELL BIOLOGY | 2001年 / 11卷 / 07期

关键词：

D O I：

10.1016/S0962-8924(01)02027-X

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Evanescent wave microscopy, also termed total internal reflection fluorescence microscopy (TIR-FM), has shed new light on important cellular processes taking place near the plasma membrane. For example, this technique can enable the direct observation of membrane fusion of synaptic vesicles and the movement of single molecules during signal transduction. There has been a recent surge in the popularity of this technique with the advent of green-fluorescent protein (GFP) as a fluorescent marker and new technical developments. These technical developments and some of the latest applications of TIR-FM are the subject of this review.

引用

页码：298 / 303

页数：6

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