Detecting single stranded DNA with a solid state nanopore

被引：342

作者：

Fologea, D

Gershow, M

Ledden, B

McNabb, DS

Golovchenko, JA

Li, JL ^{[1
]}

机构：

[1] Univ Arkansas, Dept Phys, Fayetteville, AR 72701 USA

[2] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[3] Univ Arkansas, Dept Biol Sci, Fayetteville, AR 72701 USA

[4] Harvard Univ, Div Engn & Appl Sci, Cambridge, MA 02138 USA

来源：

NANO LETTERS | 2005年 / 5卷 / 10期

关键词：

D O I：

10.1021/nl051199m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than similar to 11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

引用

页码：1905 / 1909

页数：5

共 23 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].