A T-type calcium channel required for normal function of a mammalian mechanoreceptor

被引：119

作者：

Shin, JB ^{[1
]}

Martinez-Salgado, C ^{[1
]}

Heppenstall, PA ^{[1
]}

Lewin, GR ^{[1
]}

机构：

[1] Max Delbruck Ctr Mol Med, Growth Factors & Regenerat Grp, D-13092 Berlin, Germany

来源：

NATURE NEUROSCIENCE | 2003年 / 6卷 / 07期

关键词：

D O I：

10.1038/nn1076

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The dorsal root ganglia (DRG) contain a variety of mechanoreceptors, but no molecular markers uniquely identify specific mechanoreceptor subtypes. We have used DNA microarrays and subtracted cDNA libraries to isolate genes that are specifically expressed by one type of mouse mechanoreceptor. The T-type calcium channel Ca(v)3.2 was exclusively expressed in the DRG by D-hair receptors, a very sensitive mechanoreceptor. Pharmacological blockade of T-type calcium channels indicated that this channel may be essential for normal D-hair receptor excitability including mechanosensitivity. This is the first evidence that a calcium channel is required for normal function of a vertebrate mechanoreceptor.

引用

页码：724 / 730

页数：7

共 45 条

[1] PERIPHERAL NERVOUS SYSTEM-SPECIFIC GENES IDENTIFIED BY SUBTRACTIVE CDNA CLONING [J].

AKOPIAN, AN ;

WOOD, JN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (36) :21264-21270

[2]

Brown A.G., 1981, ORG SPINAL CORD

[3] A QUANTITATIVE STUDY OF CUTANEOUS RECEPTORS AND AFFERENT FIBRES IN CAT AND RABBIT [J].

BROWN, AG ;

IGGO, A .

JOURNAL OF PHYSIOLOGY-LONDON, 1967, 193 (03) :707-&

[4] RECEPTOR TYPES IN CAT HAIRY SKIN SUPPLIED BY MYELINATED FIBERS [J].

BURGESS, PR ;

PETIT, D ;

WARREN, RM .

JOURNAL OF NEUROPHYSIOLOGY, 1968, 31 (06) :833-&

[5] Mechanosensitivity of N-type calcium channel currents [J].

Calabrese, B ;

Tabarean, IV ;

Juranka, P ;

Morris, CE .

BIOPHYSICAL JOURNAL, 2002, 83 (05) :2560-2574

[6] The vanilloid receptor: A molecular gateway to the pain pathway [J].

Caterina, MJ ;

Julius, D .

ANNUAL REVIEW OF NEUROSCIENCE, 2001, 24 :487-517

[7] Cloning and characterization of α1H from human heart, a member of the T-type Ca2+ channel gene family [J].

Cribbs, LL ;

Lee, JH ;

Yang, J ;

Satin, J ;

Zhang, Y ;

Daud, A ;

Barclay, J ;

Williamson, MP ;

Fox, M ;

Rees, M ;

Perez-Reyes, E .

CIRCULATION RESEARCH, 1998, 83 (01) :103-109

[8] Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries [J].

Diatchenko, L ;

Lau, YFC ;

Campbell, AP ;

Chenchik, A ;

Moqadam, F ;

Huang, B ;

Lukyanov, S ;

Lukyanov, K ;

Gurskaya, N ;

Sverdlov, ED ;

Siebert, PD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :6025-6030

[9] A diverse family of GPCRs expressed in specific subsets of nociceptive sensory neurons [J].

Dong, XZ ;

Han, SK ;

Zylka, MJ ;

Simon, MI ;

Anderson, DJ .

CELL, 2001, 106 (05) :619-632

[10] Nomenclature of voltage-gated calcium channels [J].

Ertel, EA ;

Campbell, KP ;

Harpold, MM ;

Hofmann, F ;

Mori, Y ;

Perez-Reyes, E ;

Schwartz, A ;

Snutch, TP ;

Tanabe, T ;

Birnbaumer, L ;

Tsien, RW ;

Catterall, WA .

NEURON, 2000, 25 (03) :533-535

← 1 2 3 4 5 →