The in vitro response to human fibroblast-derived extracellular matrix proteins is restricted by specific HLA class II genes - Relevance for coeliac disease

被引:3
作者
Jalava, T
Maki, M
Marttinen, A
Partanen, J
Koskimies, S
机构
[1] FINNISH RED CROSS & BLOOD TRANSFUS SERV, HELSINKI 00310, FINLAND
[2] UNIV TAMPERE, INST MED TECHNOL, FIN-33101 TAMPERE, FINLAND
[3] UNIV TAMPERE, SCH MED, FIN-33101 TAMPERE, FINLAND
[4] TAMPERE UNIV HOSP, DEPT PEDIAT, TAMPERE, FINLAND
基金
芬兰科学院; 英国医学研究理事会;
关键词
D O I
10.1016/0198-8859(96)00043-2
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Coeliac disease is an immunologic disease of the small intestine which is caused by ingestion of wheat gliadin, the disease-promoting agent. The disease associates strongly with the particular HLA type, HLA-DQA1*0501, DQB1*0201 alleles. Further specific autoantibodies against reticulin and endomysium are found in patients; these autoantibodies appear to be disease specific. An extracellular matrix noncollagenous protein reacts specifically with CD patients' serum immunoglobulin A and is the target of antireticulin antibodies. In this study the immune response to this matrix protein was analyzed in vitro in normal, healthy individuals. Our study shows that the immune response to Fb-CDAP is strictly regulated by the HLA-DR3, DQA1*0501, DQB1*0201 alleles, and that only those cells which were positive for these alleles produced an immune response. On the other hand, half of the cells positive for these HLA alleles were responders. Monoclonal antibodies to DR and DQ inhibited the response in an additive way, showing that both DR and DO can act as an antigen-presenting structure. The immune response to gliadin has been shown io associate with the same HLA type as CD, bur the association is not as strong. Our results show that the immune response to Fb-CDAP can be generated in vitro in genetically predisposed persons in the absence of Cn.
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页码:106 / 112
页数:7
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