Effect of ginsenoside Rd on nitric oxide system induced by lipopolysaccharide plus TNF-α in C6 rat glioma cells

被引：25

作者：

Choi, SS

Lee, JK

Han, EJ

Han, KJ

Lee, HK

Lee, J

Suh, HW

机构：

[1] Hallym Univ, Coll Med, Dept Pharmacol, Chunchon 200702, Kangwon Do, South Korea

[2] Hallym Univ, Inst Nat Med, Chunchon 200702, Kangwon Do, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2003年 / 26卷 / 05期

关键词：

ginsenoside Rd; lipopolysaccharide; TNF-alpha; nitric oxide; C6 glioma cell; ERK; Jun B; Fra-1;

D O I：

10.1007/BF02976694

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Effects of ginsenosides on nitric oxide (NO) production induced by lipopolysaccharide plus TNF-alpha (LNT) were examined in C6 rat glioma cells. Among several ginsenosides, ginsenoside Rd showed a complete inhibition against LNT-induced NO production. Ginsenoside Rd attenuated LNT-induced increased phosphorylation of ERK. Among several immediate early gene products, only Jun B and Fra-1 protein levels were increased by LNT, and ginsenoside Rd attenuated Jun B and Fra-1 protein levels induced by LNT. Furthermore, LNT increased AP-1 DNA binding activities, which were partially inhibited by ginsenoside Rd. Our results suggest that ginsenoside Rd exerts an inhibitory action against NO production via blocking phosphorylation of ERK, in turn, suppressing immediate early gene products such as Jun B and Fra-1 in C6 glioma cells.

引用

页码：375 / 382

页数：8

共 42 条

[1] THE ROLE OF JUN, FOS AND THE AP-1 COMPLEX IN CELL-PROLIFERATION AND TRANSFORMATION [J].