Rapid identification of Candida spp. in peritonitis patients by Raman spectroscopy

被引:39
作者
Ibelings, MS
Maquelin, K
Endtz, HP
Bruining, HA
Puppels, GJ
机构
[1] Univ Med Ctr Rotterdam, Erasmuc MC, Dept Gen Surg, Ctr Opt Diagnost & Therapy, NL-3015 GD Rotterdam, Netherlands
[2] Univ Med Ctr Rotterdam, Erasmuc MC, Surg Intens Care Unit, NL-3015 GD Rotterdam, Netherlands
[3] Univ Med Ctr Rotterdam, Erasmuc MC, Dept Med Microbiol & Infect Dis, NL-3015 GD Rotterdam, Netherlands
关键词
Candida spp; identification; Raman spectroscopy; rapid identification method; peritonitis; spectroscopy;
D O I
10.1111/j.1469-0691.2005.01103.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
This prospective study evaluated Raman spectroscopy for the identification of clinically relevant Candida spp. in peritonitis patients. A Raman database was developed by measuring spectra from 93 reference strains belonging to ten different Candida spp. Clinical samples were obtained from the surgical department and intensive care unit of a tertiary university hospital. In total, 88 peritoneal specimens from 45 patients with primary, secondary or tertiary peritonitis were included. Specimens were cultured initially on a selective Sabouraud medium that contained gentamicin to suppress bacterial growth. For conventional identification, a chromogenic medium was used for presumptive identification, followed by use of the Vitek 2 system for definitive identification (requiring a total time of 48-96 h). Raman measurements were taken on overnight cultures from Sabouraud-gentamicin medium. Thirty-one samples were positive for Candida by culture. Using multivariate statistical analyses, a prediction accuracy of 90% was obtained for Raman spectroscopy, which appears to offer an accurate and rapid (12-24 h) alternative for the identification of Candida spp. in peritonitis patients. The reduced turnaround time is of great clinical importance for the treatment of critically ill patients with invasive candidiasis in intensive care units.
引用
收藏
页码:353 / 358
页数:6
相关论文
共 40 条
[1]   Management of invasive candidal infections: Results of a prospective, randomized, multicenter study of fluconazole versus amphotericin B and review of the literature [J].
Anaissie, EJ ;
Darouiche, RO ;
AbiSaid, D ;
Uzun, O ;
Mera, J ;
Gentry, LO ;
Williams, T ;
Kontoyiannis, DP ;
Karl, CL ;
Bodey, GP .
CLINICAL INFECTIOUS DISEASES, 1996, 23 (05) :964-972
[2]  
BECKSAGUE CM, 1993, J INFECT DIS, V167, P1247, DOI 10.1093/infdis/167.5.1247
[3]  
CALANDRA T, 1989, LANCET, V2, P1437, DOI 10.1016/S0140-6736(89)92043-6
[4]  
COHEN J, 1994, INTENS CARE MED, V20, P522, DOI 10.1007/BF01711909
[5]   Predictive factors of mortality due to polymicrobial peritonitis with Candida isolation in peritoneal fluid in critically ill patients [J].
Dupont, H ;
Paugam-Burtz, C ;
Muller-Serieys, C ;
Fierobe, L ;
Chosidow, D ;
Marmuse, JP ;
Mantz, J ;
Desmonts, JM .
ARCHIVES OF SURGERY, 2002, 137 (12) :1341-1346
[6]   Fluconazole prophylaxis prevents intra-abdominal candidiasis in high-risk surgical patients [J].
Eggimann, P ;
Francioli, P ;
Bille, J ;
Schneider, R ;
Wu, MM ;
Chapuis, G ;
Chiolero, R ;
Pannatier, A ;
Schilling, J ;
Geroulanos, S ;
Glauser, MP ;
Calandra, T .
CRITICAL CARE MEDICINE, 1999, 27 (06) :1066-1072
[7]   Yeast identification in the clinical microbiology accepted 15 August 2000 laboratory: phenotypical methods [J].
Freydiere, AM ;
Guinet, R ;
Boiron, P .
MEDICAL MYCOLOGY, 2001, 39 (01) :9-33
[8]   Evaluation of the VITEK 2 system for rapid identification of yeasts and yeast-like organisms [J].
Graf, B ;
Adam, T ;
Zill, E ;
Göbel, UB .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1782-1785
[9]   NEW AND EMERGING YEAST PATHOGENS [J].
HAZEN, KC .
CLINICAL MICROBIOLOGY REVIEWS, 1995, 8 (04) :462-&
[10]   Comparison of the Rapid Yeast Plus panel with the API20C yeast system for identification of clinically significant isolates of Candida species [J].
Heelan, JS ;
Sotomayor, E ;
Coon, K ;
D'Arezzo, JB .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (05) :1443-1445