Accumulation of FoxP3-expressing CD4+CD25+ T cells with distinct chemokine receptors in synovial fluid of patients with active rheumatoid arthritis

被引:98
作者
Jiao, Z. [1 ]
Wang, W. [2 ]
Jia, R. [3 ]
Li, J. [1 ]
You, H. [1 ]
Chen, L. [1 ]
Wang, Y. [1 ]
机构
[1] Jiangsu Univ, Affiliated Hosp, Dept Lab Med, Zhenjiang 212001, Peoples R China
[2] Jiangsu Univ, Sch Med Technol, Dept Microbiol & Immunol, Zhenjiang 212001, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Ophthalmol, Shanghai 200030, Peoples R China
关键词
D O I
10.1080/03009740701482800
中图分类号
R5 [内科学];
学科分类号
1002 [临床医学]; 100201 [内科学];
摘要
Objective: To explore the presence and characteristics of FoxP3-expressing CD4(+)CD25(+) regulatory T cells in synovial fluid (SF) of patients with active rheumatoid arthritis (RA). Methods: The frequency and chemokine receptors expression profile of FoxP3-expressing CD4(+)CD25(+) regulatory T cells in SF and peripheral blood (PB) from RA patients and PB from healthy controls were investigated by flow cytometry using three- or four-colour intracellular staining. Results: The frequency of CD4(+)CD25(+) FoxP3(+) T cells was increased significantly in SF compared with paired PB from RA patients and PB from healthy controls (p < 0.05). However, the frequency in PB from RA patients was significantly lower than in PB from healthy controls (p < 0.05). Notably, CD4(+)CD25(+)FoxP3(+) T cells in SF expressed increased levels of inflammation-related trafficking chemokine receptors, such as CCR4, CCR5, and CXCR4. Conclusion: There is an accumulation of FoxP3-expressing regulatory T cells in RA SF, and such recruitment may be dependent on the distinct chemokine receptors expressed on regulatory T cells.
引用
收藏
页码:428 / 433
页数:6
相关论文
共 33 条
[1]
ARNETT FC, 1987, ARTHRITIS RHEUM, V31, P315
[2]
FOXP3 identifies regulatory CD25brigntCD4+ T cells in rheumatic joints [J].
Cao, D. ;
Borjesson, O. ;
Larsson, P. ;
Rudin, A. ;
Gunnarsson, I. ;
Klareskog, L. ;
Malmstrom, V. ;
Trollmo, C. .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2006, 63 (06) :444-452
[3]
Isolation and functional characterization of regulatory CD25brightCD4+ T cells from the target organ of patients with rheumatoid arthritis [J].
Cao, D ;
Malmström, V ;
Baecher-Allan, C ;
Hafler, D ;
Klareskog, L ;
Trollmo, C .
EUROPEAN JOURNAL OF IMMUNOLOGY, 2003, 33 (01) :215-223
[4]
CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease [J].
Cao, DJ ;
van Vollenhoven, R ;
Klareskog, L ;
Trollmo, C ;
Malmström, V .
ARTHRITIS RESEARCH & THERAPY, 2004, 6 (04) :R335-R346
[5]
CD4+CD25bright regulatory T cells actively regulate inflammation in the joints of patients with the remitting form of juvenile idiopathic arthritis [J].
de Kleer, IM ;
Wedderburn, LR ;
Taams, LS ;
Patel, A ;
Varsani, H ;
Klein, M ;
de Jager, W ;
Pugayung, G ;
Giannoni, F ;
Rijkers, G ;
Albani, S ;
Kuis, W ;
Prakken, B .
JOURNAL OF IMMUNOLOGY, 2004, 172 (10) :6435-6443
[6]
Evolving concepts of rheumatoid arthritis [J].
Firestein, GS .
NATURE, 2003, 423 (6937) :356-361
[7]
Foxp3 Programs the Development and Function of CD4+CD25+ Regulatory T Cells (Reprinted from vol 4, pg 330-336, 2003) [J].
Fontenot, Jason D. ;
Gavin, Marc A. ;
Rudensky, Alexander Y. .
JOURNAL OF IMMUNOLOGY, 2017, 198 (03) :986-992
[8]
Where do T cells stand in rheumatoid arthritis? [J].
Fournier, C .
JOINT BONE SPINE, 2005, 72 (06) :527-532
[9]
The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors [J].
Hirahara, Kazuki ;
Liu, Luzheng ;
Clark, Rachael A. ;
Yamanaka, Kei-ichi ;
Fuhlbrigge, Robert C. ;
Kupper, Thomas S. .
JOURNAL OF IMMUNOLOGY, 2006, 177 (07) :4488-4494
[10]
Control of regulatory T cell development by the transcription factor Foxp3 [J].
Hori, S ;
Nomura, T ;
Sakaguchi, S .
SCIENCE, 2003, 299 (5609) :1057-1061